Leaf blade anatomical study of ferns at Tham Jong Ang Waterfall in Sakaerat Environmental Research Station, Wang Nam Khieo District, Nakhon Ratchasima Province were conducted from May 2015 to April 2016. Eighteen species including fourteen genera and eight families were studied. The purpose of this research was to determine the vegetative anatomy for the identification of ferns. The specimens were investigated by leaf epidermal peeling or clearing and transverse sectioning of lamina and examination by light microscope. The generalized anatomical characteristics in all species studied are as follow: 1) epidermal cells are jigsaw-like or polygonal, anticlinal walls are straight, straight and slightly curve, rough and concave; 2) shapesof epidermal cell in transverse section are quadrangular or irregular, with 1-2 layers; 3) stomata are typical type, usually presence in the abaxial, stomatal index are 9.45-28.57 and the patterns of stoma are paracytic, pericytic, diacytic, anisocytic and anomocytic stomata; 4) trichomes are unicellular or multicellular trichomes; 5) some species are presence air space in the mesophyll; 6) mesophyll of most species studied consist of similarly palisade and spongy cells which presence more than one layer of irregular or globose in shapes; 7) shapes of main veins are curve on both sides, straight on both sides, straight on the upper and curve on the lower, v-shaped on the upper and curve on the lower and heart-shaped on the upper and curve on the lower; 8) vascular bundles in main veins are amphicribal bundle which phloem surrounding by xylem. Vascular bundle shapesare circular, triangular, oval and x-shaped with 1-3 vascular bundles; and 9) inclusions are red staining body and tannin. Using these characteristics an anatomical key was constructed to assist identification of the species. However, these anatomical characteristics are not shared between species in the family which cannot be used to support the classification. 

วันที่ตีพิมพ์
• 2017-09-29

ชื่อวารสาร
• วารสารวิทยาศาสตร์บูรพา

DOI

The  objective  of  this  study  was  to  isolate  and  characterize  lytic  phages  capable  of  infecting extended  spectrum  beta-lactamase Escherichia  coli (ESBL-E.coli).  Bacteriophages  were  isolated  from water  and  wastewater  samples  using  an  enrichment protocol  and  the  double  agar  layer  method.  Seven bacteriophages  from  different  water  samples  were  detected.  They  were  named  EP1,  EP2,  EP3,  EP4, EP5,  EP6  and  EP7,  respectively.  The  host  ranges  of  the  7  bacteriophages  were  determined  by performing  spot  tests  with  17  bacterial  strains.  All  bacteriophages,  except  EP2,  infected  more  than  one bacterial  host.  Transmission  electron  microscopy  revealed  the  bacteriophages  belonged  to  the  order Caudovirales.  Bacteriophage  EP1,  EP2,  EP3,  EP5,  EP6  and  EP7  were  members  of  the  family Siphoviridae,  but  bacteriophage  EP4  belonged  to  the  family Podoviridae.  One-step  growth  experiment was  employed  to  determine  a  latent  period,  a  burst  time  and  a  burst  size  of  each  bacteriophage.  Allbacteriophages had a double-stranded DNA which was digested with EcoRI.DNA restriction patterns by agarose  gel  electrophoresis  and  protein  profiles  by  SDS-PAGE  of  the  bacteriophages  showed  the diversity among the isolated bacteriophages.

วันที่ตีพิมพ์
• 2017-07-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี

DOI
• https://li01.tci-thaijo.org/index.php/sci_ubu/article/view/97634/76037

This research aimed to develop academic achievement in topic of cell cycle and mitosis using a hands–on activity of science inquiry. Research methodology composed of 1) developing achievements test which was 9 items of 4 multiple choices test, 2) developing a 150– minutes hands–on activity of science inquiry, and 3) implementing the activity to the samples which were 79 of 10th grade students from 2 classrooms, drawn by cluster random sampling. Data were collected from pre– and post–academic achievements test and work sheets. Preand post-academic achievements, learning progression (), efficiency, and effectiveness of learning activity were analyzed. The results showed that for the first classroom, pre– and post– academic achievements were in a low level (25.44%) and excellent level (80.99%), respectively, which indicated a statistically significant increasing (p< .05).The students’ learning progression was 74.35% ( = 0.74), categorized as a high gain level. The efficiency (E1/E2) and effectiveness (E.I.) of the activity were 81.18/80.99 and 0.7435, respectively. For the second classroom, pre– and post–academic achievements were in a low level (27.64%) and excellent level (80.22%), respectively, which indicated a statistically significant increasing (p < .05). The students’ learning progression was 72.35% ( = 0.72), categorized as a high gain level.The E1/E2 and E.I. of the activity were 80.98/80.22 and 0.7235, respectively

วันที่ตีพิมพ์
• 2017-07-01

ชื่อวารสาร
• วารสารหน่วยวิจัยวิทยาศาสตร์ เทคโนโลยี และสิ่งแวดล้อมเพื่อการเรียนรู้

DOI

Comparative anatomy of the genus Jatropha L. (Euphorbiaceae) in Thailand including J. curcas L., J. gossypiifolia L., J. integerrima Jacq., J. multifida L. and J. podagrica Hook. was investigated by means of leaf epidermal peeling method, clearing method and transverse section of leaf blade, petiole and stem. Shared characteristics of the genus are 1) the U-shaped midrib, 2) the circular-shaped stem, 3) the collateral bundle, 4) the paracytic stomata and 5) the presence of laticifer. Furthermore, the anatomical characteristics of the genus Jatropha provide features which are taxonomically useful in identification at species level viz. 1) the presence/absence of trichomes, 2) different types of trichomes, 3) the distribution of stomata, 4) the shapes of leaf margins, 5) the number of palisade layers, 6) the presence/absence of fiber cells and 7) the presence/absence of papillae.

วันที่ตีพิมพ์
• 2017-07-01

ชื่อวารสาร
• วารสารพฤกษศาสตร์ไทย

DOI
• http://www.qsbg.or.th/bot/journal/showarticle_en.aspx?type=article&id=284

Bacteriophages or phages are viruses that infect bacteria. Bacteriophages are the most abundant viral entity on earth and exist in all the ecosystems. Bacteriophages were discovered over one hundred year ago. Like other types of viruses, bacteriophages vary in their shape and genetic makeups (genome). Bacteriophage genomes can consist of either DNA or RNA, surrounded by a protein coat. The capsid of a bacteriophage can be polyhedral, filamentous or head-tail structure. To start an infection, a bacteriophagevirion has to first attach onto the surface of their host cell by the recognition of receptors on its host and phage attachment protein. In host cell, bacteriophages can propagate in two  possible  life  cycles;  lytic  cycle  and  lysogenic  cycle.  InternationalCommittee on Taxonomy  of  Viruses  (ICTV) classifies the bacteriophages under an order Caudovirales according to morphology and nucleic acid type. Over 96% of bacteriophages are tailed and contain double-stranded DNA. After the discovery of bacteriophages, many researchers thought  about  the  potential  of  bacteriophages  on  killing  bacteria,  which  could  possibly  make  them  as  therapeutic agents. But most of this research effort stopped soon after the discovery of antibiotics. Today, bacteriophages have become animportant instrument to biotechnologists.  They have been exploited for various purposes like cloning vectors  for  gene  cloning,  antibacterial  agents  for  controlling  of  infection,  and  effective  diagnostics  for  detection  of bacterial pathogens. They are alsobeing used for phage display systems for revealing the peptide of interest on phage coat protein. In this review article all these applications have been summarized.

วันที่ตีพิมพ์
• 2019-09-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี

DOI

The objectives of this research were to characterize growth and melanin production on different culture media, effect of copper ion and cobalt ion on growth and melanin production including antibacterial activity of crude melanin. The results revealed that actinomycetes strain PNST-01 have good growth on ISP-7 medium, synthetic medium, while strain PRBS-12 grow very well on ISP-3 medium, complex medium. Both strains produced high amount of melanin showing brownish colony on ISP-7 medium but there is no melanin pigment production on ISP-1 medium and ISP-6 medium. Moreover, the growth rate was reduced and both strains could not produce melanin pigment on ISP-7 medium without supplement with copper chloride and cobalt chloride. Crude melanin extract of strain PNST-01 showed antimicrobial activity against Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 25923 but could not inhibit Escherichia coli, Shigella dysenteriae, Salmonella typhi, Klebsiella pneumoniae, Serratia marcescens, Pseudomonas aeruginosa and Streptococcus pyogenes. While crude melanin extract of strain PRBS-12 gave antimicrobial activity only on B. cereus ATCC 11778 and B. subtilis ATCC 6633. Based on the study, the results can be used as key information for further development of melanin production and application in various industries.

วันที่ตีพิมพ์
• 2019-07-01

ชื่อวารสาร
• วารสารวิชาการและวิจัย มทร.พระนคร

DOI

The anatomical studies of four species belonging to genus Lysiphyllum in Thailand were investigated, including L. binatum (Blanco) de Wit, L. hookeri (F. Muell.) Pedley, L. strychnifolium (Craib) A. Schmitz and L. winitii (Craib) de Wit. Samples were studied by leaf epidermis peeling and clearing methods, transverse section of leaf blade and petiole by paraffin method, section on three dimensions and macerate of wood. Common characteristics of studied species are as follow: 1) smooth cuticle 2) the presence of secretory cell in leaf epidermis 3) round and elliptic epidermal cells in transverse section 4) bifacial leaf 5) round and bend down of leaf margin 6) vascular bundle surrounded by fiber in leaf blade and petiole 7) the presence of secretory cell with dark red staining in leaf blade and petiole 8) circular shaped petiole 9) vessel exclusively solitary 10) alternate bordered intervessel pit 11) the presence of inclusion with dark red staining in vessel member and 12) druse and prismatic crystals in ray parenchyma of wood.

วันที่ตีพิมพ์
• 2019-06-01

ชื่อวารสาร
• วารสารพฤกษศาสตร์ไทย

DOI

The leaf epidermal characteristics of 26 selected species in 22 genera of Bignoniaceae were anatomically investigated using peeling and clearing methods. The general epidermal characteristics of studied species are as follows; 1) the cuticle structures are smooth and striated, 2) the shapes of epidermal cells are irregular and polygonal, 3) the cell wall are straight, emarginate and cleft, 4) the types of stomata are anomocytic, cyclocytic, diacytic and paracytic, 5) the types of trichome are peltate, unicellular, multicellular, glandular multicellular, stellate and prickle and 6) the types of inclusions are raphide crystal and red staining inclusion cell. These characteristics can be used to construct a key for species identification. Moreover, the outstanding anatomical characteristics consisting of the cuticle structures, the epidermal cell shapes, the stomatal types, and the trichome types are considered to divide the studied species into six groups. These obtained results were also in line with the molecular study of Olmstead et al. (2009) who divided this family into eight clades and six of them were found in Thailand.

วันที่ตีพิมพ์
• 2019-06-01

ชื่อวารสาร
• วารสารพฤกษศาสตร์ไทย (THAI JOURNAL OF BOTANY)

DOI

The objective of this descriptive studywere:1) to study levels of health beliefs and accident prevention behaviors ofDisaster Prevention and Mitigation Officer in Warinchamrab Local Government, 2) to study the relationship between personal factors, health beliefs with accident prevention behaviors of Disaster Prevention and Mitigation Officer in Warinchamrab Local Government. There were 32 malesDisaster Prevention and Mitigation Officer. The instrument was used questionnaire for collecting data. Descriptive statistics and Inferential statistics were used for determining the data as frequency, percentage, mean, standard deviation and Pearson’s Correlation Coefficient with statistical significance at the95% confidence level.The results of this research were the Disaster Prevention and Mitigation Officer with age 40 or over were 56.25%,had diploma education were 59.38%, married were 71.88% and working over 5 years were 78.13%. The levels of health beliefs of Disaster Prevention and Mitigation Officer was high level. ( X = 2.63, S.D = 0.51) The levels of accident prevention behaviors of Disaster Prevention and Mitigation Officer was high level. ( X = 2.42, S.D = 0.62)and found that the levels of education were significantly correlated with accident prevention behaviors. (r = 0.521, p-value = 0.002)

วันที่ตีพิมพ์
• 2019-01-01

ชื่อวารสาร
• วารสารความปลอดภัยและสิ่งแวดล้อม E-Journal

DOI

Many strains of lactic acid bacteria (LAB) have probiotic properties that can promote consumers’ health. This study aims to find LAB having probiotic properties. Ten isolates of LAB isolated from fermented foods including Nham, Pla Som and fermented vegetables were tested for their tolerance to acid and to bile salt, considered to be major probiotic properties. It was found that LAB F1, isolated from Pla Som, was the most tolerant isolate to acid at pH 2 and to bile salt at the concentration of 2%. Furthermore, the study showed that the LAB isolate had antimicrobial ability against Escherichia coli O157:H7 ATCC 43895 and Staphylococcus aureus ATCC 25923. When LAB F1 was identified using 16S rDNA sequenceanalysis, it was foundthat its16S rDNA sequenceshowed99% homology tothatofLactobacillus plantarum strain JCM 1149 (accession number NR_115605.1). LAB with probiotic properties obtained from this study can be further characterized in order to utilize it in the future.

วันที่ตีพิมพ์
• 2020-03-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยมหาสารคาม

DOI

The aim of this study was to isolate and characterize Escherichia coli-specific lytic bacteriophages from different wastewater sources.  Three bacteriophages, named JC01, JC02, and JC03, were successfully isolated from wastewater samples collected from the wastewater treatment plants of Kudprakhow community, Sanprasitthiprasong Hospital, and Khong Chiam Hospital, respectively.  Host range determination revealed that all bacteriophages had only Escherichia coli as a host, indicating a high specific host range property.  The inhibition of clinical isolates of multidrug resistant E. coli showed that bacteriophages JC01, JC02, and JC03 inhibited the growth of E. coli at the percentages of 51.7 (138/267), 52.4 (140/267), and 28.5 (76/267),  respectively.  All bacteriophages were able to tolerate normal saline and distilled water for up to 40 min but were not able to tolerate ethanol and hydrogen peroxide at every time point tested.  Heat stability showed that bacteriophage JC01 had the highest resistance at 60°C after 60 min.  Meanwhile, bacteriophages JC02 and JC03 showed resistances at 60°C after 45 min.  Bacteriophage classification by genome analysis demonstrated that all the bacteriophages were double-stranded DNA viruses.  Viral particle morphology observed by transmission electron microscope found that all bacteriophages had the viral particle composed of a head with a hexagonal shape and long tails with contractile.  The size from head to tail of all the bacteriophages was approximately 200 nm.  Therefore, based on the International Committee on Taxonomy of Viruses (ICTV) classification, bacteriophages JC01, JC02, and JC03 belonged to Family Myoviridae, Order Caudovirales.  Therefore, the bacteriophages derived through this study can be further used to study their potential use in advanced research steps, such as in cell cultures and animal models.

วันที่ตีพิมพ์
• 2020-01-01

ชื่อวารสาร
• Journal of Current Science and Technology

DOI

This descriptive study aimed to measure the concentration of airborne fungi and bacteria in five swine farms located in Si Khai district Warin Chamrap, Ubon Ratchathani and to examine the factors dealing with the growth of airborne fungi and bacteria in the indoor and outdoor e.g. temperature, relative humidity, velocity and also the density of pigs per unit area. Single stage impactor was carried out to monitor the amount of airborne fungi and bacteria and operated as follows NIOSH method 0800. It was operated at a flow rate of 28.3 L/min. Also, temperature, relative humidity, and velocity were assessed by an anemometer. The highest amount of airborne fungi in indoor air was found in the 4th swine farm (1,272.02 CFU/m 3 ) which was higher than the recommendation level of American Conference of Governmental Industrial Hygienists (ACGIH): Air Sampling Instruments for Evaluation of Atmospheric Contaminants (1995) 1,000 CFU/m 3 , whereas the concentration of total airborne bacteria at the 3rd swine farm reached to 3,494.22 CFU/m 3 . This concentration had not exceeded the recommendation level of Occupational Health and Safety Research Institute Robert Sauvé (IRSTT) equal to 10,000 CFU/m 3 in the air for 8-hour working day. Owing to statistical analysis, the results indicated that the temperature was correlated significantly with the airborne fungi concentrations in indoor air at 95% confidential interval (p-value=0.037). Other environmental factors (e.g. relative humidity, velocity and the density of pigs per unit area) had not been correlated with the airborne fungi and bacteria concentrations with statistical significance (p-value<0.05). However, an environmental surveillance and control are a great need to decrease the amount of airborne fungi and bacteria in the swine plants. 

วันที่ตีพิมพ์
• 2020-01-01

ชื่อวารสาร
• วารสารวิจัยสาธารณสุขศาสตร์ มหาวิทยาลัยขอนแก่น

DOI

วันที่ตีพิมพ์
• 2017-12-07

ชื่อวารสาร
• การประชุมวิชาการระดับชาติด้านวิทยาศาสตร์สุขภาพ ครั้งที่ 1 (The1st National Conference on Health Sciences Research and Innovation: Knowledge Transformation towards Thailand 4.0).

DOI

วันที่ตีพิมพ์
• 2017-12-07

ชื่อวารสาร
• การประชุมวิชาการระดับชาติด้านวิทยาศาสตร์สุขภาพ ครั้งที่ 1 (The1st National Conference on Health Sciences Research and Innovation: Knowledge Transformation towards Thailand 4.0)

DOI

วันที่ตีพิมพ์
• 2017-12-07

ชื่อวารสาร
• การประชุมวิชาการระดับชาติด้านวิทยาศาสตร์สุขภาพ ครั้งที่ 1 (The1st National Conference on Health Sciences Research and Innovation: Knowledge Transformation towards Thailand 4.0

DOI

วันที่ตีพิมพ์
• 2017-12-07

ชื่อวารสาร
• การประชุมวิชาการระดับชาติ ครั้งที่ 4

DOI

วันที่ตีพิมพ์
• 2017-12-07

ชื่อวารสาร
• การประชุมวิชาการระดับชาติ ครั้งที่ 4

DOI

วันที่ตีพิมพ์
• 2017-12-07

ชื่อวารสาร
• การประชุมวิชาการระดับชาติมหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน ครั้งที่ 14

DOI

วันที่ตีพิมพ์
• 2017-11-29

ชื่อวารสาร
• การประชุมวิชาการชมรมคณะปฏิบัติงานวิทยาการ อพ.สธ. ครั้งที่ 8

DOI

วันที่ตีพิมพ์
• 2017-11-29

ชื่อวารสาร
• 0

DOI

วันที่ตีพิมพ์
• 2017-11-24

ชื่อวารสาร
• 0

DOI

วันที่ตีพิมพ์
• 2017-11-06

ชื่อวารสาร
• Asia-Pacific Social Sciences Conference and International Conference on Education and Psychology

DOI

วันที่ตีพิมพ์
• 2017-11-06

ชื่อวารสาร
• International Journal of Environmental Studies

DOI

We analyzed the asymmetrical molar surfaces of two fairy shrimps, Streptocephalus sirindhornae Sanoamuang, Murugan, Weekers & Dumont, 2000 and Branchinella thailandensis Sanoamuang, Saengphan & Murugan, 2002. The peripheral region of the left and right mandibles in both species has several rows of rectangular teeth. Each row has 5–8 small stubby denticles in S. sirindhornae and 6–14 numerous, small, sharp denticles in B. thailandensis. The posteriodorsal region of the left mandible bears one row of 11 large, stout teeth and one row of six large, stout teeth, respectively. The posteriodorsal region of the right mandible in S. sirindhornae has three rows of teeth, whereas in B. thailandensis it has two rows plus a tuft of setae. The intersetular distance of filter-feeding limb endites in B. thailandensis is wider (1.37 ± 0.27 µm) than that of S. sirindhornae (0.38 ± 0.02 µm). These morphological differences affect feeding behaviour. Feeding experiments showed that B. thailandensis can consume larger starch grains than S. sirindhornae. The largest percentages of ingested food size in both immature and mature shrimps are in the 5–30 µm range. Because B. thailandensis tends to mature far earlier than cooccurring S. sirindhornae, we hypothesise that B. thailandensis adults may consume S. sirindhornae nauplii. No evidence of cannibalism was found in the two species.

วันที่ตีพิมพ์
• 2017-09-01

ชื่อวารสาร
• Journal of Crustacean Biology

DOI

This study aimed to isolate lactic acid bacteria (LAB) carrying cadmium resistant genes on plasmids from foods. A total of 30 fermented food samples were collected from local markets and supermarkets. Isolation of cadmium resistant LAB colonies was performed by spread plate technique using MRS agar medium supplemented with cadmium (MRS-Cd). One hundred and fifty cadmium resistant isolates were randomly selected and subjected to plasmid extraction. Only one isolate (LAB-V12) from a fermented vegetable harbored a single plasmid of about 9 Kb. 16S rDNA sequencing data showed that LAB-V12 was likely to be Lactobacillus plantarum. The results from plasmid curing demonstrated that a cured strain of the LAB-V12 was sensitive to cadmium. Hence, probably cadmium resistant marker is plasmid mediated. The plasmid containing cadmium resistance gene was tested for stability in its host. It was observed that the LAB-V12 could maintain the plasmid after approximately 100 generations of growth without cadmium selection. The plasmid might be further developed to be a food-grade cloning vector that is useful for genetic modification of LAB.

วันที่ตีพิมพ์
• 2018-12-01

ชื่อวารสาร
• Asian Journal of Agriculture and Biology

DOI

วันที่ตีพิมพ์
• 2018-10-29

ชื่อวารสาร
• The Congress of Science and Technology of Thailand (STT44)

DOI

วันที่ตีพิมพ์
• 2018-09-08

ชื่อวารสาร
• The 24th International Conference on Aerosol Science & Technology, 2017 Forum on Fine Particulate Matter (PM2.5) and Climate Change

DOI

วันที่ตีพิมพ์
• 2018-09-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี

DOI

วันที่ตีพิมพ์
• 2018-09-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี

DOI

วันที่ตีพิมพ์
• 2018-08-31

ชื่อวารสาร
• การประชุมวิชาการระดับชาติวิทยาลัยแพทยศาสตร์และ การสาธารณสุข ครั้งที่ 7

DOI

วันที่ตีพิมพ์
• 2018-07-12

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 12

DOI

วันที่ตีพิมพ์
• 2018-07-12

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 12

DOI

วันที่ตีพิมพ์
• 2018-07-12

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 12

DOI

วันที่ตีพิมพ์
• 2018-07-12

ชื่อวารสาร
• การประชุมวิชาการระดับชาติ มอบ. วิจัย ครั้งที่ 12

DOI

วันที่ตีพิมพ์
• 2018-07-12

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 12

DOI

วันที่ตีพิมพ์
• 2018-03-01

ชื่อวารสาร
• Asian Journal of Microbiology, Biotechnology and Environmental Sciences

DOI

วันที่ตีพิมพ์
• 2018-03-01

ชื่อวารสาร
• Research Journal of Pharmaceutical, Biological and Chemical Sciences

DOI

วันที่ตีพิมพ์
• 2018-02-20

ชื่อวารสาร
• Asian Journal of Microbiology, Biotechnology and Environmental Sciences

DOI

วันที่ตีพิมพ์
• 2018-02-13

ชื่อวารสาร
• Pakistan Journal of Biotechnology

DOI

วันที่ตีพิมพ์
• 2018-01-30

ชื่อวารสาร
• การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 56 "ศาสตร์เกษตรสู่เศรษฐกิจสร้างสรรค์"

DOI

วันที่ตีพิมพ์
• 2018-01-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี

DOI

วันที่ตีพิมพ์
• 2018-01-01

ชื่อวารสาร
• วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี

DOI

A total of 73 yeast strains were screened for an extracellular β-glucosidase producing ability by using substrate p-nitrophenyl-β-D-glucoside (pNPG). Among them strain JK9/1 showed the maximum β-glucosidase activity which was further identified as Candida sp. strain JK9/1 based on ITS1-5.8S-ITS2 and D1/D2 domains of the subunit (26S) ribosomal DNA. Optimum conditions for β-glucosidase production were determined using different biophysical and biochemical parameters. The maximum -glucosidase production was obtained in the optimum medium containing 1.5% (w/v) of xylose as a carbon source and 0.02 % (w/v) KNO3 as additional inorganic nitrogen, with an initial medium pH of 4.0 and at 25oC for 6 days of cultivation. Under optimal conditions, Candida sp. strain JK9/1 produced the enzyme of 7.21 ± 0.19 U.mL-1, which was 4.2 times higher than the amount before optimization (1.71±0.03 U.mL-1). This can contribute its β-glucosidase producing ability for a great potential to be used in the biotechnological application.

วันที่ตีพิมพ์
• 2019-12-01

ชื่อวารสาร
• Malaysian Journal of Biochemistry & Molecular Biology

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• เอกสารสืบเนื่องจากการประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13, มหาวิทยาลัยอุบลราชธานี, หน้า

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• เอกสารสืบเนื่องจากการประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• เอกสารสืบเนื่องจากการประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• เอกสารสืบเนื่องจากการประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13, มหาวิทยาลัยอุบลราชธานี, หน้า

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13

DOI

วันที่ตีพิมพ์
• 2019-07-11

ชื่อวารสาร
• การประชุมวิชาการ มอบ.วิจัย ครั้งที่ 13

DOI

วันที่ตีพิมพ์
• 2019-05-01

ชื่อวารสาร
• Journal of Cleaner Production

DOI

วันที่ตีพิมพ์
• 2019-04-01

ชื่อวารสาร
• Indian Journal of Science and Technology

DOI

วันที่ตีพิมพ์
• 2019-03-30

ชื่อวารสาร
• การประชุมวิชาการนาเสนอผลงานวิจัยระดับบัณฑิตศึกษา (Symposium) ครั้งที่ 11 บัณฑิตวิทยาลัย

DOI

วันที่ตีพิมพ์
• 2019-03-30

ชื่อวารสาร
• การประชุมวิชาการนาเสนอผลงานวิจัยระดับบัณฑิตศึกษา (Symposium) ครั้งที่ 11 บัณฑิตวิทยาลัย มหาวิทยาลัยราชภัฏอุบลราชธานี

DOI

Toxin-antitoxin (TA) systems regulate fundamental cellular processes in bacteria and represent potential therapeutic targets. We report a new RES-Xre TA system in multiple human pathogens, including 
Mycobacterium tuberculosis. The toxin, MbcT, is bactericidal unless neutralized by its antitoxin MbcA. To investigate the mechanism, we solved the 1.8 Å-resolution crystal structure of the MbcTA complex. We found that MbcT resembles secreted NAD+-dependent bacterial exotoxins, such as diphtheria toxin. Indeed, MbcT catalyzes NAD+ degradation in vitro and in vivo. Unexpectedly, the reaction is stimulated by inorganic phosphate, and our data reveal that MbcT is a NAD+ phosphorylase. In the absence of MbcA, MbcT triggers rapid M. tuberculosis cell death, which reduces mycobacterial survival in macrophages and prolongs the survival of infected mice. Our study expands the molecular activities employed by bacterial TA modules and uncovers a new class of enzymes that could be exploited to treat tuberculosis and other infectious diseases.

วันที่ตีพิมพ์
• 2019-03-21

ชื่อวารสาร
• Molecular Cell

DOI

Important ‘floral’ aromas naturally occur in grapes predominantly as flavourless glycoconjugate precursors. Since these aroma compounds can be released by hydrolysis, different glycosidase enzymes can potentially contribute different aromas to wines. In this paper, the effects of crude and purified Candida easanensis JK8 β-glucosidases on wine aroma precursors of Muscat of Alexandria grape powder were investigated by GC/MS combined with stir bar sorptive extraction (SBSE). A total of 19 bound volatile compounds were identified, including phenols, terpenes, aldehyde, ester and alcohols. The concentrations of terpenes especially nerol and geraniol, and β-Damascenone, a C13-norisoprenoid, contributes flowery and slightly fruity aromas were significantly increased in enzyme treated. These results suggest the potential application of this yeast β-glucosidase as an aroma-enhancing enzyme in winemaking.

วันที่ตีพิมพ์
• 2019-12-01

ชื่อวารสาร
• Natural Product Research

DOI

 Heat and high light intensity affected physiology and morphology of young Dipterocarpus alatus Roxb. leaf studied. D. alatus is a native forest tree and being extended to cultivation in the field as an economic crop. Nowadays, climate change due to increasing in temperature and light intensity can affect growth, morphological and photosynthetic traits in D. alatus. This research aimed to study the effects of high temperature and strong light intensity on physiology and morphology of the young D. alatus. The experiment was decided in CRD with 5 replications. The two-year-old D. alatus was treated with combination stress between temperature (at 35°C or 41°C) and light intensity (at 700 or 1800 μmol m-2s-1) for 7 days. Plant morphology, gas exchange, PSII efficiency and photosynthetic pigment contents were measured. Strong light intensity (1800 μmol m-2s-1) affected plant morphology by leaf burning and heat injury. However, high temperature (41°C) combined with strong irradiation enlarged leaf injury and also increased percentage of heat injury (3.01±0.81%; T41L1800) compared to control (0.07±0.00%; T35L700). In contrast, it reduced percentages of leaf angle (-8.77±2.82%) and leaf area (-1.04±0.38%). In addition, the combination stress influenced reduction of net photosynthetic rate and contents of Chl a+b and Chl a but unaffected Chl b and Car contents. Therefore, combined stress affected young D. alatus by damaging photosynthetic pigments such as Chl a and injured leaf tissue. This resulted in reduction in both of photosynthetic mechanism and D. alatus leaf growth. Thus, young D. alatus leaf (two-year-old) was susceptible to heat combined with excessive light.

วันที่ตีพิมพ์
• 2020-05-01

ชื่อวารสาร
• Journal of Tropical Agricultural Science

DOI

Asian megacities are undergoing rapid population and infrastructure growth, which is resulting in significant air quality problems linked to atmospheric fine particles (PM2.5). This study focuses on characterizing carbonaceous aerosols in an urban area of Bangkok, Thailand. The Microclimate and Air Pollutants Monitoring tower is located on the edge of Kasetsart University campus and was used to perform vertical measurements. Mass concentration data were collected using area dust monitors (ADR1500) at different levels above the ground (30 m, 75 m, and 110 m) during two time periods, daytime (08:00– 19:00) and nighttime (20:00–07:00), throughout the monsoon season in 2015. All relevant micrometeorological parameters were simultaneously monitored. Backward air mass trajectories were calculated using HYSPLIT to assess the possible external pollution contributions. The thermal optical transmittance (TOT) method following the NIOSH 870 protocol was used to determine amounts of elemental carbon (EC) and organic carbon (OC) in samples. A data analysis showed average PM2.5 values at observation heights of 30 m, 75 m, and 110 m of 6.21 ± 2.45, 8.12 ± 3.65, and 9.03 ± 3.93 µg m–3, respectively; corresponding OC concentrations of 4.13 ± 2.65, 4.01 ± 4.07, and 4.11 ± 3.58 µg m–3, respectively, and EC concentrations of 1.02 ± 0.84, 1.07 ± 0.95, and 0.50 ± 0.70 µg m–3, respectively. The results show distinct gradients of increasing concentrations of PM2.5 with increasing elevation in contract with the OC and EC concentrations which decrease with height. 

วันที่ตีพิมพ์
• 2020-01-01

ชื่อวารสาร
• Aerosol and Air Quality Research

DOI

Emerging consumers have driven the transformation of lifestyles and consumption patterns in Thailand to resemble industrialised countries. In Thailand, food waste generated from the consumption of consumers (plate waste) has become problematic and the number of research is still limited. This research conceptualises Thai consumers' roles to address plate waste and quantifies plate waste at university canteens for assessing and analysing socio-ethical impacts. Waste hierarchy and citizen-consumer concepts are used to analyse consumers' roles and to guide the analysis on socio-ethical impacts of plate waste. The canteens at Ubon Ratchathani University (UBU) became the investigation site. It is revealed that bringing in the citizen-consumer concept broadens what Thai consumers can do to address food waste, offering potentials to promote sustainable food provision systems and consumption practices. For the socio-ethical aspect, it is found that a week of plate waste from about 3,800 consumers could feed 116 Thai adults for one day.

วันที่ตีพิมพ์
• 2020-10-01

ชื่อวารสาร
• International Journal of Sustainable Society

DOI
• https://www.inderscienceonline.com/doi/abs/10.1504/IJSSOC.2020

Emerging consumers have driven the transformation of lifestyles and consumption patterns in Thailand to resemble industrialised countries. In Thailand, food waste generated from the consumption of consumers (plate waste) has become problematic and the number of research is still limited. This research conceptualises Thai consumers' roles to address plate waste and quantifies plate waste at university canteens for assessing and analysing socio-ethical impacts. Waste hierarchy and citizen-consumer concepts are used to analyse consumers' roles and to guide the analysis on socio-ethical impacts of plate waste. The canteens at Ubon Ratchathani University (UBU) became the investigation site. It is revealed that bringing in the citizen-consumer concept broadens what Thai consumers can do to address food waste, offering potentials to promote sustainable food provision systems and consumption practices. For the socio-ethical aspect, it is found that a week of plate waste from about 3,800 consumers could feed 116 Thai adults for one day.

วันที่ตีพิมพ์
• 2021-01-04

ชื่อวารสาร
• International Journal of Sustainable Society

DOI
• 10.1504/IJSSOC.2020.112445

This study aims to explore ways to minimize plate waste at university canteens by studying plate waste and consumers at three main canteens of a university, Thailand. Using university canteens in Thailand as a case, plate waste was characterized and quantified and consumers’ insights concerning food consumption practices were examined through focus group discussion. The results revealed that each consumer wasted edible food around 19 grams/meal. The generation of plate waste is affected by the food provision system, including canteen setting, food purchasing procedure and food quality. In addition, the presence of stray dogs in the canteens inhibited consumers from finishing up their food. Thus, improving the food provision system is crucial to engage consumers in achieving zero plate waste.

วันที่ตีพิมพ์
• 2021-11-01

ชื่อวารสาร
• International Journal of Sustainability in Higher Education

DOI
• https://doi.org/10.1108/IJSHE-02-2021-0056

Rice straw is a major agricultural waste that can be used as an alternative substrate to expensive raw materials for endoglucanases (CMCase) production by microorganisms. This study aimed to search for a microorganism having the potential to produce endoglucanase from rice straw. From compost samples, 40 bacterial colonies were isolated on carboxymethylcellulose (CMC) agar. Among them, 16 isolates showed a hydrolysis zone on a CMC agar plate with hydrolysis (HC) values ranging from 1.15±0.02 to 4.40±0.52. Based on hydrolysis zone diameter and HC value, isolates CP1, CP2 and CP3 were further examined for their CMCase production in CMC broth. According to CMCase production and stability, isolate CP1 was selected for further study. The optimal pH and temperature for CMCase production of isolate CP1 were 5 and 45 °C, respectively. When using pre-treated rice straw as a substrate for semisolid-state fermentation, the highest CMCase activity of 0.142 ± 0.008 U/mL was obtained in a medium containing pre-treated rice straw of 60 g/L. The sequence alignment analysis and phylogenetic analysis suggested that the isolate CP1 was likely to be Streptomyces thermocoprophilus. The microorganism obtained from this study may be not only industrially important but also beneficial to the environment.

วันที่ตีพิมพ์
• 2021-12-01

ชื่อวารสาร
• Journal of Pure and Applied Microbiology

DOI